rabbit polyclonal phosphorylated s6 ribosomal protein  (Cell Signaling Technology Inc)

Journal: bioRxiv

Article Title: The odor of a nontoxic tetrodotoxin analog, 5,6,11-trideoxytetrodotoxin, is detected by specific olfactory sensory neurons of the green spotted puffers but is processed in overlapping olfactory bulbar area with food odors

doi: 10.1101/2023.08.22.552781

Figure Lengend Snippet: Differential activation areas in the olfactory bulb by TDT and AAs. Immunohistochemistry employing anti-pS6 antibody on transverse OB sections exposed to the vehicle, AAs, and TDT. ( A ) Five vertical lines ( 1-5 ) in the sagittal brain diagram represent the rostral-caudal positions of transverse right OB sections, which are correspondingly exposed to the vehicle( B ), AAs( C ), and TDT( D ). Four fish examined for each condition showed similar results. Scale bar = 100 µm. The density quantification of pS6-positive neurons in the dorsomedial ( dm ) and ventrolateral ( vl ) part of each section was measured (n = 4; white squares in D5 correspond to an area of 10 4 µm 2 ). The rostral-caudal transition of pS6-immunopositive cell density labeled in the dm and vl part of the OB by odorant stimulations. Graphs are divided by the presented odors ( E ) or divided by dm or vl ( F ). The same lowercase letters denote non-significant differences and different letters represent a significant difference by two-factor repeated measure ANOVA with Holm correction (P < 0.05). Abbreviations : Tel , telencephalon; OB , olfactory bulb; d , dorsal; v , ventral; l , lateral; m , medial..

Article Snippet: Odorant-responsive cells were labeled with an anti-phosphorylated ribosomal protein S6 (pS6) rabbit polyclonal antibody (Ser244, Sers247 anti-pS6, 44-923G, Thermo Fisher Scientific, MA; 1:5,000), which was used in mouse OSNs ( ) and green spotted puffer OSNs , as a neural activity marker.

Techniques: Activation Assay, Immunohistochemistry, Labeling

Journal: The Journal of Physiology

Article Title: Muscle regeneration is improved by hot water immersion but unchanged by cold following a simulated musculoskeletal injury in humans

doi: 10.1113/jp287777

Figure Lengend Snippet: Figure 9. Representative blots and quantification of phosphorylated mTOR (A, B), phosphorylated-S6 (C, D) and phosphorylated-p70 (E, F) protein expression at PRE, D5 and D11 in CWI, TWI and HWI groups Data are presented as medians along with individual data. #Significantly different from PRE (P < 0.05); ##significantly different from PRE (P < 0.01); †significantly different from D5 (P < 0.05); ∗significantly different between groups (P < 0.05).

Article Snippet: The membranes were incubated overnight at 4°C with the following primary antibodies: rabbit polyclonal phosphorylated nuclear factor-κB (NF-κB) p65 (1:1000, cat. no. 3033, Cell Signaling Technology, Danvers, MA, USA), monoclonal rat interleukin (IL)-10 (1:500, sc-73309, Santa Cruz Biotechnology, Dallas, TX, USA), mouse monoclonal HSP70 (1:750, ADI-SPA-810, Enzo, Farmingdale, NY, USA), mouse monoclonal HSP27 (1:750, ADI-SPA-800, Enzo Life Sciences, Farmingdale, NY, USA), rabbit polyclonal phosphorylated mammalian target of rapamycin (mTOR; 1:1000, cat. no. 2971, Cell Signaling Technology), rabbit polyclonal phosphorylated S6 ribosomal protein (1:1000, cat. no. 5364, Cell Signaling Technology), mousemonoclonal p-p70 S6 kinase (1:1000, cat. no. 9206, Cell Signaling Technology) rabbit polyclonal vascular endothelial growth factor (VEGF; 1:500, sc507, Santa Cruz Biotechnology) and rabbit polyclonal transforming growth factor β (TGF-β 1:1000, cat. no. 3711, Cell Signaling Technology, Danvers, MA, USA).

Techniques: Expressing